a rapid diagnostic system for the
ultra-sensitive detection of pathogens.
system is based on the CANARY® (Cellular Analysis and Notification of Antigen Risks and Yields) technology,
a revolutionary diagnostic technology
developed by the scientists at Massachusetts
Institute of Technology and recently
published in Science.BioFlash® delivers extremely
rapid detection of pathogens at previously
unseen levels of sensitivity and specificity.
Accurate results can be obtained in
less than 3 minutes by non-expert
FIG. 1 — CANARY®
The patented, self-contained
system consists of engineered biosensors
expressing membrane bound, pathogen-specific
antibodies and a calcium sensitive
bioluminescent molecule. Cross-linking
of the antibodies by even minute amounts
of the specific pathogen leads to
elevation of intracellular calcium
and light emission (FIG.1). The amplified
light output is detected using an
- Ultra-sensitive detection of
pathogens in less than 3 minutes.
- Combination of speed and sensitivity
unmatched by current methods.
EASY TO USE
- Simple protocol requiring minimal
instrumentation and training.
- Validated high-fidelity detection
- PCR-like signal amplification
of speed and sensitivity of the BioFlash®
system is unmatched by other methods such
as immunoassays and PCR. Rapid immunoassays
require at least 15 minutes, but have a
much higher limit of detection. Ultra-fast PCR still requires at least 30
minutes of sample preparation, notwithstanding
PCR contamination issues.For example, the BioFlash® system
offers detection down to 50 colony forming
units (cfu) of pathogenic Escherichia
coli O157:H7 without cross-reaction
with normal E. coli strains (FIG.2). These results compare very favorably
with publications describing the use of
PCR to detect bacteria in food (LOD of 10-10,000
cfu/g without overnight culture as an enrichment
FIG. 2 — BioFlash® Readout
Automatic sample collection and analysis: The BioFlash-E™ biological aerosol collection and analysis is a very rapid process (FIG. 3) and is accomplished in less than 3 minutes.
FIG. 3 — Automated assay process for biological aerosol samples
Instrumentation: The BioFlash-E™
Biological Identifier is a portable device occupying approximately one cubic foot and is simple to use.